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Banu, N.
- Inhibition of Antibiofilm Mediated Virulence Factors in Pseudomonas aeruginosa by Andrographis Paniculata
Authors
1 Department of Biotechnology, Vels Institute of Science, Technology & Avanced Studies. Chennai, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 1 (2017), Pagination: 141-144Abstract
The focal intent of the study is about Quorum sensing (QS) which is well known for biofilm production, bacterial virulence and antibiotic resistance. Inhibition of quorum sensing in bacteria can reduce the risk of pathogenicity in all bacterial infection. In the present study the methanolic leaf extract of Andrographis paniculata has showed its anti quorum sensing activity against acyl homoserine lactone -dependent showed a concentration dependent (10-100 μg/ml) reduction in biofilm production in Pseudomonas aeruginosa and the inhibition level was upto 10-80%. Microtiter plate (MTP)assay and Light microscopic analysis has further confirmed the antibiofilm activity in Pseudomonas aeruginosa when treated with methanolic leaf extract of Andrographis paniculata. In addition the methanolic ectract of Andrographis paniculata itself showed no antibacterial activity in Pseudomonas aeruginosa.Keywords
Antibiofilm, Quorum Sensing Inhibition, Acyl Homoserine Lactone, Pseudomonas aeruginosa, Microtiter Plate, Andrographis paniculata.- A First Report on the Antiproliferative activity of Sodium Copper Chlorophyllin from Endangered Medicinal Plant Rhinacanthus nasutus on HepG2 and HeLa Cell Lines
Authors
1 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Vels University, Velan Nagar, P.V. Vaithiyalingam Road, Pallavaram, Chennai – 117, Tamil Nadu, IN
2 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies (VISTAS), VELS University, Velan Nagar, P.V. Vaithiyalingam Road, Pallavaram, Chennai - 117, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 1 (2017), Pagination: 325-329Abstract
Plants are the richest source of bioactive compounds and they have been used as medicine also. Chlorophyllin, a water soluble derivative of chlorophyll (chl) in which magnesium has been replaced with copper and the phytol chains lost. Chlorophyllin has been used by human population for over 50 years for medicinal purposes with no adverse effects. Chlorophyllin is a promising chemopreventive agent to block cancer primarily by inhibiting carcinogen such as AFB1. The objective was to extract the bioactive pigment chloropyllin from Rhinacanthus nasutus and characterize by IR and NMR and evaluate the effect of chlorophyllin on inhibition of cell proliferation in HepG2 and HeLa cell lines. The HepG2 and HeLa cell lines was exposed with different concentrations of chlorophyllin, and found that there was a significant dose dependent reduction in cell viability. The IC50 value at 48Hrs was 62.5μg/ml for Rhinacanthus nasutus Chlorophyllin on HepG2 Cell line and HeLa cell line. This is the first report of chlorophyllin from fresh leaves of Rhinacanthus nasutus on HepG2 and HeLa cell lines. It also suggests the benefit of these natural compounds against HepG2 and HeLa cell lines.Keywords
Sodium Copper Chlorophyllin, Rhinacanthus nasutus, HepG2 Cell Lines, HeLa Cell Lines.- Antiquorum Sensing Potential of Andrographolide from Andrographis paniculata in Vibrio harveyi
Authors
1 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies, Chennai, IN
2 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies, Chennai, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 2 (2017), Pagination: 449-452Abstract
The recently discovered cell-cell communication system through signaling molecules among bacterial pathogens is known as Quorum sensing. It is well known for bacterial virulence, antibiotic resistance and biofilm production. Microbial pathogenicity is reduced by inhibiting the quorum sensing activity in all infections caused by bacteria. In this present study the compound Andrographolide which is present as a secondary metabolite in Andrographis paniculata was assessed for its anti quorum sensing activity against acyl homoserine lactone (AHL)-dependent biofilm formation and bioluminescence production in Vibrio harveyi (MTCC 7771). The Andrographolide showed a concentration dependent (100 μg/ml) reduction in biofilm production to a level of 97% and reduction of bioluminescence at the level of 98% in Vibrio harveyi (MTCC 7771) and compared with the standard Andrographolide (sigma aldrich). Light microscopic analysis and Microtiter plate assay further confirmed the reduction in the biofilm forming ability of Vibrio harveyi (MTCC 7771) when treated with andrographolide. The andrographolide at its standard concentration showed antibacterial effect on Vibrio harveyi(MTCC 7771).Keywords
Andrographolide, Andrographis paniculata, Vibrio harveyi, Bioluminescence, Microtiter Plate Assay.- Free Radical Scavenging Activity and Total Antioxidant Capacity of Tin Chlorophyllin from Morinda citrifolia L
Authors
1 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Vels University, Chennai, Tamil Nadu, IN
2 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Vels University, Chennai, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 2 (2017), Pagination: 453-455Abstract
Medicinal plants are the rich source of harmless medicines, and used for the treatment of various diseases for thousands of years. They can provide biologically active molecules and lead structures for development of modified derivatives with enhanced activity or reduced activity. Green plants synthesize and preserve a variety of biochemical products, many of which are extractable and used as chemical feed stocks or as raw material for several scientific investigations. Tin chlorophyllin, a water soluble sodium salt of Chlorophyll is one among the family of phytochemical compounds. The central metal atom, magnesium of chlorophyll has been replaced with tin and the phytol chains lost in chlorophyllin. The antioxidant activity of tin chlorophyllin from the leaves of Morinda citrifolia L. was investigated by using in vitro antioxidant models such as DPPH scavenging assay and Phosphomolybdenum method. The free radical scavenging activity of tin chlorophyllin on the stable radical 1, 1- diphenyl-2-picrylhydrazyl (DPPH) was evaluated and the percentage of inhibition was determined. By phosphomolybdenum method, the total antioxidant capacity of tin chlorophyllin was determined and the absorbance values were expressed as the number of gram equivalent of ascorbic acid. The results demonstrate the potential antioxidant activity of tin chlorophyllin and support its possible role in human health protection and disease prevention.Keywords
Tin Chlorophyllin, Morinda citrifolia L., DPPH, Free Radicals, Phosphomolydenum.- A Review on the Bioactivity of Various Metallochlorophyllin-A Chlorophyll Derivative
Authors
1 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Vels University, IN
2 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Vels University, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 2 (2017), Pagination: 480-486Abstract
Medicinal plants are the rich source of harmless medicines, and used for the treatment of various diseases for thousands of years. They can provide biologically active molecules and lead structures for development of modified derivatives with enhanced activity or reduced activity. The isolation and identification of active principles and elucidation of the mechanism of action of a drug is of paramount importance. Hence, works in both mixture of traditional medicine and single active compounds are very important. One such compound is metallochlorophyllin, a water soluble analogue of the ubiquitous green pigment chlorophyll. Metallochlorophyllin can neutralize several physically relevant oxidants and may decrease oxidative damage induced by chemical carcinogens and radiation. It also acts as an effective inhibitor of aflatoxin hepatocarcinogenesis in animal models by blocking carcinogen bioavailability. Further anti-cancer effects of metallochlorophyllin including antioxidant activity, inhibition of enzymatic activity that converts inert procarcinogens into active carcinogens; stimulation of enzymatic activity that promotes the elimination of toxic substances from the body; protection from radiation; and antitumor activity have likewise been evidenced by controlled studies. The metallochlorophyllins are compounds worthy of further study, with the potential to provide great benefit to human health.Keywords
Chlorophyll, Metallochlorophyllin, Anti-Oxidant, Anti-Microbial, Anti-Carcinogenic.- Evaluation of Antibacterial Activity of Sodium Copper Chlorophyll in from Mimosa pudica L
Authors
1 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies, Chennai, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 2 (2017), Pagination: 487-489Abstract
Synthetic water soluble form of sodium copper salt of chlorophyll is Chlorophyll in. Antibacterial efficiency of Cholorophyllin from Mimosa pudica was tested against Escherichia coli, Staphylococcus aureus, proteus vulgaris, Klebsiella pneomoniae, Bacillus subtilis, and pseudomonas arogenosa. Agar well diffusion method was used to determine the Antibacterial activity and minimum inhibitory concentration (MIC). Sodium copper chlorophyll in from Mimosa pudica shows significant antibacterial activity against all tested micro organisms. Similarly standard sodium copper chlorophyll in also exhibited significant activity. The minimum zone of inhibition and comparatively greater inhibitory concentration were determined in Escherichia coli, Staphylococcus aureus and showed significant activity in other tested organisms. The Spectrum of activity observed in the present study may be the leaf extracts of sodium copper chlorophyll in from Mimosa pudica could be a possible source to obtain new and effective herbal medicines to treat infectious diseases.Keywords
Mimosa pudica, Sodium Copper Cholorophyllin, MIC, Antibacterial Activity.- Anti-Proliferative Activity of Chlorophyllin from Phyllanthus emblica L. Against MCF-7 and Vero Cell Line
Authors
1 Department of Biotechnology, Mohamed Sathak College of Arts and Science, Sholinganallur, Chennai- 119, IN
2 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Vels University, Pallavaram, Chennai, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 2 (2017), Pagination: 516-524Abstract
Chlorophyllin refers to any one of a group of closely related water-soluble salts that are semi-synthetic derivatives of chlorophyll. It is most common form is sodium copper derivative used as a food additive and in alternative medicine. In this study sodium copper chlorophyllin was extracted from Phyllanthus emblica, a medicinal plant and estimated by visible spectrophotometer and further characterized by TLC, IR, NMR and GC-Mass Chromatographic techniques and studied the anticancer activity against MCF-7 cell line and Vero cell line. Result shows that Chlorophyllin of Phyllanthus emblica has good anticancer activity for breast cancer cell line by using MTT assay.Keywords
Chlorophyllin, Thin Layer Chromatography, MTT Assay.- Anti Fungal Activity of Metallochlorophyllins from Leucas aspera L
Authors
1 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Vels University, Chennai, Tamil Nadu, IN
2 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies(VISTAS), Vels University, Chennai, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 2 (2017), Pagination: 513-515Abstract
Metallochlorophyllin belongs to a group of compound, porphyrins containing a chelated metal ion in the center of the molecule. The central metal atom consists of metals like copper, magnesium, zinc etc with loss of phytol chain. The present study was to extract the metallochlorophyllin from Leucas aspera L. and to check its antifungal activity. The result shows that the metallochlorophyllins (Sodium copper chlorophyllin, Sodium magnesium chlorophyllin, Sodium zinc chlorophyllin) of Leucas aspera L. has significant anti fungal activity against Aspergillus niger and Rhizopus microsporus with Amphotericin-B (Std) 20 μg /ml as positive control. The active zone of inhibition of Sodium copper chlorophyllin was around 7-15 mm, Sodium magnesium chlorophyllin 15-22 mm, Sodium zinc chlorophyllin 6-10 mm against the pathogenic fungi. This result confirms that the medicinal value of metallochlorophyllins of Leucas aspera L. as an anti fungal sources.Keywords
Metallochlorophyllins, Antifungal, Medicinal Plants.- Screening of Antimicrobial Activity of tin Chlorophyllin from Morinda citrifolia L
Authors
1 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Vels University, Chennai, Tamil Nadu, IN
2 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Vels University, Chennai, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 3 (2017), Pagination: 693-695Abstract
Metallochlorophyllin, a water soluble analogue of the ubiquitous green pigment chlorophyll belongs to a group of compounds, porphyrins that contain a cheated metal ion in the center of the molecule. Tin chlorophyll in is the metallochlorophyllin with tin as its central metal ion and the phytol chains lost. The aim of the present study was to explore the antimicrobial activity of tin chlorophyll in from the leaves of Morinda citrifolia L. The leaves were collected and the tin chlorophyll in was extracted. The extracted tin chlorophyll in was subjected for antimicrobial study by disc diffusion method. The test organisms include Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Salmonella typhi, Pseudomonas aeroginosa, Aspergillus niger, Rhizopus microsporus. The inhibition zones were detected and were calculated for the various organisms. The Minimal Inhibitory Concentration (MIC) was also determined and found to be 125μg/ml (Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeroginosa) and 250μg/ml (Escherichia coli, Salmonella typhi). The results of the present study conclude that tin chlorophyll in from the leaves of Morinda citrifolia L. showed significant antimicrobial activity.Keywords
Metallochlorophyllin, Tin Chlorophyll in, Antibacterial, Antifungal, MIC.- Inhibition of Biofilm formation in Serratia marcescens by Andrographolide from Andrographis paniculata
Authors
1 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies, Chennai, IN
2 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies, Chennai, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 3 (2017), Pagination: 789-791Abstract
Quorum sensing (QS) is well known for bacterial virulence, antibiotic resistance and biofilm production. Inhibition of quorum sensing can reduce the risk of microbial pathogenicity in all infection. In this present study the compound Andrographolide which is present in Andrographis paniculata leaf extract was assessed for its anti quorum sensing activity against acyl homoserine lactone (AHL)-dependent biofilm formation in Serratia marcescens. The Andrographolide obtained from Andrographis paniculata exhibited a concentration dependent (100 μg/ml) reduction in biofilm production in Serratia marcescens to a level of 90% and compared with the standard Andrographolide (sigma aldrich). Microtiter plate and Light microscopic analysis further confirmed the reduction in the biofilm forming ability of Serratia marcescens when treated with andrographolide. In addition the Andrographolide present in Andrographis paniculata by itself showed no growth inhibitory effect on Serratia marcescens.
Keywords
Andrographis paniculata, Quorum Sensing Inhibition, Acyl homoserine lactone, Serratia marcescens, anti Bacterial Assay, Antibiofilm.- Antibacterial activity of Sodium Copper Chlorophyllin (SCC) from Leucas aspera L
Authors
1 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Vels University, Chennai, Tamil Nadu, IN
2 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies(VISTAS), Vels University, Chennai, Tamil Nadu, IN